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Northern blot analysis of S. mutans UA159 and its RelA-deficient strains (TJ-relA and JL-ΔrelA). Cells were grown in complete FMC (+) or amino acid-depleted FMC (−) for 45 and 90 min. RNA (10 μg per lane) was isolated, separated in a 0.9% formaldehyde gel, transferred to a nylon membrane, and hybridized to probes specific for elongation factor G (A) and ribosomal L1 (sll) (B) genes.
