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Expression of relA mRNA and RelA from S. mutans UA159 in response to amino acid starvation. (A) Slot blot of total RNA; (B) Western blot analysis of RelA levels with an anti-RelSeq polyclonal antibody diluted 1:500. RNAs (10 μg per lane) or total protein (10 μg per lane) was isolated from samples grown in complete FMC (1), in FMC minus supplemented amino acids for 45 min (2), and in FMC minus supplemented amino acids 90 min (3) and from biofilm (4) or planktonic (5) cells grown in BM.
