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. 2004 Mar;72(3):1512–1518. doi: 10.1128/IAI.72.3.1512-1518.2004

FIG. 2.

FIG. 2.

SAPK/JNK phosphorylation and kinase activity is significantly increased following uptake of L. pneumophila AA100. (A) Representative Western blot of JNK phosphorylation over a 60-min time course. Infected MDMs (U, untreated; A, AA100; G, GL10; and E, E. coli) were analyzed for SAPK/JNK phosphorylation and kinase activity. (B) JNK kinase activity measured by determining increase in phosphorylation of recombinant protein substrate (c-Jun) through Western blot analysis; representative Western blot of kinase assay showing increased activity in AA100 at 60 min. (C) Graphic representation of data averaged from multiple experiments showed significant increase in JNK activity in AA100 over GL10 (P = 0.025) (*) and over E. coli (P = 0.014) (**) (N = 4).