Table 2.
Comparison of Papp values of uncoated liposomes, chitosan-TGA77-coated liposomes, chitosan-TGA150-coated liposomes and chitosan-TGA150-MNA-coated liposomes. Enhancement ratios result from the comparison of each test solution with the corresponding control solution. Indicated values represent the means ± SD of at least three experiments (*p < 0.05, **p < 0.01 and ***p < 0.001 compared with each buffer control). Additionally the effect of named test compounds was tested on the TEER. Table shows the decrease in TEER and the merit index, which is defined as the ratio of the increase in Papp to the decrease in the TEER.
| Substrate | Test compound | Papp × 10− 6 [cm/s] | Fold increase in Papp | Fold decrease in TEER | Merit index |
|---|---|---|---|---|---|
| FD4 | Buffer 37 °C | 1.98 ± 0.36 | – | – | – |
| Uncoated liposomes | 3.79 ± 0.64 | 1.9* | 1.2 | 1.5 | |
| CS-TGA77-coated liposomes | 5.45 ± 0.47 | 2.8** | 1.5 | 1.9 | |
| CS-TGA150-coated liposomes | 6.06 ± 0.20 | 3.1*** | 1.5 | 2.1 | |
| CS-TGA150-MNA-coated liposomes | 8.07 ± 0.73 | 4.1*** | 1.6 | 2.6 | |
| Rho-123 | Buffer 37 °C | 1.89 ± 0.06 | – | – | – |
| Buffer 4 °C | 2.65 ± 0.03 | 1.4 | – | – | |
| Uncoated liposomes | 2.95 ± 0.22 | 1.6* | – | – | |
| CS-TGA77-coated liposomes | 5.29 ± 0.77 | 2.8* | – | – | |
| CS-TGA150-coated liposomes | 6.42 ± 0.82 | 3.4* | – | – | |
| CS-TGA150-MNA-coated liposomes | 7.94 ± 0.95 | 4.2* | – | – |