Table 3.
Comparison of the absorptive and secretory apparent permeability coefficients (Papp) of Rho-123 and resulting efflux ratios in the presence of different test compounds. Indicated values represent the means ± SD of at least three experiments. Additionally the effect of named test compounds was tested on the TEER (AP to BL direction).
| Substrate | Test compound | Absorptive (AP to BL) Papp × 10− 6 [cm/s] | Secretory (BL to AP) Papp × 10− 6 [cm/s] | Efflux ratio (secretory Papp/absorptive Papp) | Fold decrease in TEER |
|---|---|---|---|---|---|
| Rho-123 | Buffer | 1.86 ± 0.78 | 6.22 ± 2.07 | 3.3 | – |
| Verapamil 100 μM (apical) | 3.49 ± 0.86 | 5.47 ± 0.99 | 1.6 | 1.1 | |
| CS-TGA150-MNA-coated liposomes (apical) | 2.77 ± 0.24 | 5.01 ± 0.74 | 1.8 | 1.5 | |
| CS-TGA150-MNA coated liposomes plus verapamil 100 μM (apical) | 3.54 ± 0.51 | 3.58 ± 1.66 | 1.0 | 1.6 |