Immunoblot analysis of whole-cell proteins prepared from strains χ3306 (dnaK+), CS2635 (dnaK+/pTKY608), CS2021 (dnaK::Cm), CS2501 (dnaK::Cm/pTKY608), CS2069 (dnaK::Cm, Ts+ suppressor), and CS2773 (dnaK::Cm/pTKY608, Ts+ suppressor) with an antiserum against either DnaK protein (A) or DnaJ protein (B). Plasmid pTKY608 contains the complete dnaK-dnaJ operon of S. enterica serovar Typhimurium χ3306. p+, pTKY608; Ts+, Ts+ suppressor.