Table 1.
serial number | Name | Primers |
---|---|---|
PrimercDNA |
TMV-cDNA |
5’-TCGACATAGGGACATCTTC-3’ |
Primer1a,c |
WT-TMV-CP-FOR |
5’-GGAATTCCATATGTCTTACAGTATCACTACTCC-3’ |
Primer2b,d |
WT-TMV-CP-REV |
5’- CCGCTCGAGTCAAGTTGCAGGACCAGAGG-3’ |
Primer3b |
TR-TMV-CP-FOR |
5’-CGGGATCCATGTCTTACAGTATCACTACTCC-3’ |
Primer6a,d,e |
TR-His-TMV-CP-FOR |
5’-GGAATTCCATATGATCACTACTCCATCACAGTTCG-3’ |
Primer8e |
TR-His-TMV-CP-REV |
5’-CCGCTCGAGTCAACCAGAGGTCCAAACCAA-3’ |
Primer9c | TR-His-TMV-CP-REV | 5’-CCGCTCGAGTCAAGTTGCAGGACCAGAGG-3’ |
aThe WT-His-TMV-CP12 fragment was amplified by using primer1 and primer2.
bThe WT-GST-TMV-CP32 fragment was amplified by using primer3 and primer2.
cThe TR-His-TMV-CP19 fragment was amplified by using primer1and primer9.
dThe TR-His-TMV-CP62 fragment was amplified by using primer6 and primer2.
eThe TR-His-TMV-CP68 fragment was amplified by using primer6 and primer8.