Skip to main content
. 2012 Nov 21;9:279. doi: 10.1186/1743-422X-9-279

Table 1.

DNA sequences of the primers

serial number Name Primers
PrimercDNA
TMV-cDNA
5’-TCGACATAGGGACATCTTC-3’
Primer1a,c
WT-TMV-CP-FOR
5’-GGAATTCCATATGTCTTACAGTATCACTACTCC-3’
Primer2b,d
WT-TMV-CP-REV
5’- CCGCTCGAGTCAAGTTGCAGGACCAGAGG-3’
Primer3b
TR-TMV-CP-FOR
5’-CGGGATCCATGTCTTACAGTATCACTACTCC-3’
Primer6a,d,e
TR-His-TMV-CP-FOR
5’-GGAATTCCATATGATCACTACTCCATCACAGTTCG-3’
Primer8e
TR-His-TMV-CP-REV
5’-CCGCTCGAGTCAACCAGAGGTCCAAACCAA-3’
Primer9c TR-His-TMV-CP-REV 5’-CCGCTCGAGTCAAGTTGCAGGACCAGAGG-3’

aThe WT-His-TMV-CP12 fragment was amplified by using primer1 and primer2.

bThe WT-GST-TMV-CP32 fragment was amplified by using primer3 and primer2.

cThe TR-His-TMV-CP19 fragment was amplified by using primer1and primer9.

dThe TR-His-TMV-CP62 fragment was amplified by using primer6 and primer2.

eThe TR-His-TMV-CP68 fragment was amplified by using primer6 and primer8.