Table 2.
Protein building blocks used or referenced herein
| Building block | Origin | Abbreviation |
|---|---|---|
| Wild type TMV coat protein |
N. tabacum K326 |
WT-TMV-CPa |
| Wild type TMV coat protein with GST tags |
E. coliBL21(DE3)-RIL |
WT-GST-TMV-CP32b |
| Wild type TMV coat protein with His tags |
E. coliBL21(DE3)-RIL |
WT-His-TMV-CP12c |
| Truncated 19 TMV coat protein with His tags |
E. coliBL21(DE3)-RIL |
TR-His-TMV-CP19d |
| Truncated 62 TMV coat protein with His tags |
E. coliBL21(DE3)-RIL |
TR-His-TMV-CP62e |
| Truncated 68 TMV coat protein with His tags | E. coliBL21(DE3)-RIL | TR-His-TMV-CP68f |
a The wild type TMV-CP was purified from N. tabacum K326.
bThe wild type TMV-CP expressed and purified from reconstructed prokaryotic expression vector PGEX-6P-1-TMV-CP32 that was expressed in competent cell BL21(DE3)-RIL.
cThe wild type TMV-CP expressed and purified from reconstructed prokaryotic expression vector pET28a-TMV-CP12 that was expressed in competent cell BL21(DE3)-RIL.
dThe truncated TMV-CP expressed and purified from reconstructed prokaryotic expression vector pET28a-TMV-CP19 that was expressed in competent cell BL21(DE3)-RIL.
eThe truncated TMV-CP expressed and purified from reconstructed prokaryotic expression vector pET28a-TMV-CP62 that was expressed in competent cell BL21(DE3)-RIL.
fThe truncated TMV-CP expressed and purified from reconstructed prokaryotic expression vector pET28a-TMV-CP68 that was expressed in competent cell BL21(DE3)-RIL.