Skip to main content
. 2012 Nov 21;9:279. doi: 10.1186/1743-422X-9-279

Table 3.

The crystallization solutions of ammonium sulfate

Sample Protein concentration
and buffera 		Crystallization
bufferb 		Temperature
(K)
WT-TMV-CP32 (cleaved GST)
 8 –14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0
 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5
 277/295
WT-His-TMV-CP12
 8–14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0
 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5
 277/295
TR-His-TMV-CP19
 8–14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0
 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5
 277/295
TR-His-TMV-CP68
 8–14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0
 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5
 277/295
TR-His-TMV-CP62 8–14 mg/mL, 20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0 0.10–0.35 mol/L ammonium sulfate, 0.1 mol/L Tris buffer, pH 6.5–8.5 277/295

a The buffers were provided with filter membrane.

b The buffers were provided with filter membrane.