Table 4.
Examples of TR-His-TMV-CP19 crystals in Figure5
| Examples | Protein Concentration | Crystal Conditions | Crystal appearance | Resolutions |
|---|---|---|---|---|
| A |
8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.1 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 6.8 |
The typical dodecahedral crystals were rapid growth (approximately 24 hr) at 295 K |
>7.0 Å |
| B |
8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.1 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.5. |
The irregular octahedral crystals were rapid growth (approximately 24 hr) at 295 K |
-- |
| C |
8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.1 mol/L ammonium sulfate and 0.2 mol/L Tris/HCl, pH 7.0. |
The irregular octahedral crystals were rapid growth (approximately 24 hr) at 295 K |
-- |
| D |
8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.3 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. |
The typical octahedral crystals were rapid growth (approximately 24 hr) at 295 K |
-- |
| E |
8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 6.8. |
The octahedral crystals were rapid growth (approximately 24 hr) at 295 K |
-- |
| F |
8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. |
The octahedral crystals were rapid growth (approximately 24 hr) at 295 K |
-- |
| G |
8.0 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 6.8. |
The Octahedral crystals were rapid growth (approximately 24 hr) at 295 K |
>5.0 Å |
| H |
14 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.3 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 8.0. |
The splintered off and crowded together crystals were rapid growth (approximately 24 hr) at 295 K |
-- |
| I |
22 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.3 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. |
The hexahedral octahedral crystals were Slow growth (approximately 7 d) at 295 K |
-- |
| J |
14 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.25 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. |
The typical octahedral crystals were Slow growth (approximately 1-2 d) at 295 K |
2.9–3.0 Å |
| K |
22 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) |
0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. |
The dodecahedral crystals were slow growth (approximately 24 hr) at 295 K |
-- |
| L | 22 mg/mL (20 mmol/L sodium phosphate buffer, 100 mmol/L sodium chloride, pH 8.0) | 0.2 mol/L ammonium sulfate and 0.1 mol/L Tris/HCl, pH 7.7. | The dodecahedral crystals were Slow growth (approximately 9d) at 295 K | -- |
- stand for the crystals which didn't been diffraction by X-ray methods.