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Detection of virus receptor using virus histochemistry with Envision® system and 3,3’-diaminobenzidine as substrate which gives a brown colour at the binding site in Atlantic salmon primary gill epithelial cells incubated at room temperature for 1 h with ISA viral antigen (a). Negative control cells (b) NaOH treated (i.e. saponification) prior to incubation with ISA viral antigen.
