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. 2004 Mar;72(3):1275–1283. doi: 10.1128/IAI.72.3.1275-1283.2004

FIG. 2.

FIG. 2.

Nuclear run-on assay of iNOS and IP-10 in THP-1 macrophages. (A) Representative nuclear run-on assay of PMA-differentiated THP-1 cells. Probes for the IP-10, iNOS, the plasmid pGEM as a negative control, and the GAPDH housekeeping gene are shown. Lane 1, untreated; lane 2, infected with a clinical strain of M. tuberculosis (MOI of 3:1) for 3 days; lane 3, treated with 1 ng of IFN-γ/ml for 2 h; lane 4, both infected with M. tuberculosis and treated with IFN-γ. (B) Cumulative data from six separate experiments with THP-1 macrophages for iNOS (left) and IP-10 (right) nuclear transcription. IP-10 transcription was significantly induced by M. tuberculosis (#, P = 0.01) and IFN-γ (†, P ≤ 0.04 compared to results with no treatment and TB treatment), and results with the combination were significantly increased compared to results with either one alone and with untreated cells §, P < 0.02). Values are expressed as means ± SEM.