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2D NMR spectra of metabolites extracted from LPC-treated plants, upon exposure to freezing stress of −2.5°C for 24 h: (A) 2D NMR TOCSY spectrum showing characteristic proton correlations in unsaturated fatty acids and (B) 2D NMR HSQC spectrum showing characteristic proton and carbon signals of unsaturated fatty acids and sugars or sugar alcohols.
