Figure 5.

Sub-cellular localization of NNV capsid protein and BiP protein in NNV-infected GF-1 cells. Grouper fin cell line GF-1 was infected with NNV at MOI of 10. At 24 hr post-infection, the cells were fixed and co-stained with rabbit anti-NNV capsid and mouse anti-BiP, followed by Texas Red conjugated anti-rabbit IgG and Fluorscein conjugated mouse IgG. The Nucleus was visualized by counter-staining with 4', 6-diamidino-2-phenylindole (DAPI). The NNV capsid protein (A) is shown to co-localize with BiP protein (B) in distinct area within the cytoplasm as indicated by the merged image (C). Uninfected GF1 cell was indicated by white arrow.