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Suppression of the I.11E phenotype by deletion of PA5097 (hutT). LDH release by RAW264.7 macrophages infected with P. aeruginosa PAO1, a mutant with an in-frame deletion of popB, the transposon insertion mutant I.11E, as well as a mutant of PAO1 combining the I.11E insertion with an in-frame deletion of PA5097 (hutT). The infections were carried out in complete RPMI tissue culture medium (which contains histidine) supplemented with glutamine and 10% FBS.
