TABLE 2.
Populations of purified CD4+ T lymphocytes that traverse HUVEC are enriched for cells that secrete IFN-γ
| Donor | % of CD4+ T lymphocytes secreting IFN-γ
|
|||
|---|---|---|---|---|
| Initial population | Migrated across unstimulated HUVECa | Migrated across IL-1β-treated HUVECab | Migrated across B. burgdorferi-treated HUVECa,b | |
| B | 30 | 62 | 53 | 62 |
| E | 26 | 51 | 38 | 49 |
| F | 12 | 39 | 29 | 34 |
All migrated populations showed significant enrichment for cells that secrete IFN-γ compared to the initial population (P < 0.05).
On average, 2.3 ± 0.8-fold more T lymphocytes traversed stimulated HUVEC than unstimulated endothelium in these experiments.