TABLE 4.
Populations of T lymphocytes that traverse HUVEC are neither enriched for nor depleted of T cells that secrete IL-4
| Donor | Type of T cells addeda | % of T lymphocytes secreting IL-4
|
|||
|---|---|---|---|---|---|
| Initial population | Migrated across unstimulated HUVEC | Migrated across IL-1β-treated HUVECb | Migrated across B. burgdorferi-treated HUVECb | ||
| B | CD4+ | 9.0 | 8.5 | 6.6 | 5.7 |
| E | CD4+ | 4.7 | 6.9 | 6.2 | 6.3 |
| F | CD4+ | 3.1 | 8.3 | 4.5 | 4.4 |
| B | CD8+ | 3.3 | 5.1 | 5.8 | 3.3 |
| C | CD8+ | 3.9 | 0.7 | 0.8 | 1.3 |
| G | CD8+ | 2.3 | 3.4 | —c | 1.7 |
| D | Unfractionated (CD4+, CD8+) | 1.5 (1.2, 0.4) | 2.9 (1.0, 1.9) | 2.1 (1.5, 0.6) | 1.9 (1.4, 0.5) |
a
Assays were performed with unfractionated T lymphocytes or purified CD4+ or CD8+ T cells. For the study in which unfractionated T cells were added, the percentages of IL-4-secreting cells are reported for the total populations of T lymphocytes and for the CD4+ and CD8+ subsets.
b
On average, 2.2 ± 0.7-fold more T lymphocytes traversed stimulated HUVEC than unstimulated endothelial monolayers in these experiments.
c
The endothelium was not activated by IL-1β in this experiment, as assessed by comparing the numbers of T cells that migrated across untreated versus IL-1β-treated HUVEC.