Table 1.

Primers and probes used to detect TSPyV and MCPyV

Virus	Primers & probes sequence (5′-->3′)	Region	Amplicon size
MCPyV	FWD-CCACAGCCAGAGCTCTTCCT	LT	140
	REV-TGGTGGTCTCCTCTCTGCTACTG
	FAM-TCCTTCTCAGCGTCCCAGGCTTCA-TAMRA
MCPyV	FWD-TGCCTCCCACATCTGCAAT	VP1	59
	REV-GTGTCTCTGCCAATGCTAAATGA
	6FAM-TGTCACAGGTAATATC-MGBNFQ
TSPyV	FWD-TGTGTTTGGAAACCAGAATCATTTG	LT	140
	REV-TGCTACCTTGCTATTAAATGTGGAG
	FAM-TTCTTCTTCCTCCTCATCCTCCACCTCAAT-BHQ1
TSPyV	FWD-AGTCTAAGGACAACTATGGTTACAG	VP1	140
	REV-ATTACAGGTTAGGTCCTCATTCAAC
	FAM-ACAGCAGTGACCAGGACAAGCCTACTTCTG-BHQ1
Tail Sequence	AACTGACTAAACTAGGTGCCACGTCGTGAAAGTCTGACAAGTGTCTCTGCCAA TGCTAAATGA