TABLE 1.
Primers used for Q-RT-PCR of bovine cytokines and for generation of standards
| Gene | Primera | Sequence | Primer for standard | Standard size (bp) |
|---|---|---|---|---|
| IFN-γ | F | TGGAGG ACTTCA AAAAGC TGATT | AGCCAA ATTGTC TCCTTC TACTTC | 261 |
| IFN-γ | R | TTTATG GCTTTG CGCTGG AT | CTGACT TCTCTT CCGCTT TCTG | |
| IL-1α | F | TTGGTG CACATG GCAAGT G | GAACGA CGCCCT CAATCA AA | 475 |
| IL-1α | R | GCACAG TCAAGG CTATTT TTCCA | GGGCTC GACTCC TTCAT | |
| IL-1β | F | GCCTTCAATAACTGTGGAACCAAT | NDb | ND |
| IL-1β | R | GTATATTTCAGGCTTGGTGAAAGGA | ND | |
| IL-2 | F | CCTCAA CTCCTG CCACAA TGTA | CCTCAA CTCCTG CCACAA TGTA | 376 |
| IL-2 | R | GTTTGC AACGAG TGCAAG AGTTA | CCCTGT AGTTCC AAAACG ATTCTC | |
| IL-4 | F | GCCACA CGTGCT TGAACA AA | CATTGT TAGCGT CTCCTG GTA | 403 |
| IL-4 | R | TGCTTG CCAAGC TGTTGA GA | GCTCGT CTTGGC TTCATT C | |
| IL-5 | F | TGGTGG CAGAGA CCTTGA CA | CTGGTG GCAGAG ACCTTG ACACT | 320 |
| IL-5 | R | GAATCA TCAAGT TCCCAT CACCTA | CAACTT TCCATT GTCCAC TCTGTG | |
| IL-6 | F | GGCTCC CATGAT TGTGGT AGTT | AGCGCA TGGTCG ACAAAA TCT | 523 |
| IL-6 | R | GCCCAG TGGACA GGTTTC TG | GCCCAG TGGACA GGTTTC TG | |
| IL-8 | F | GGAAAA GTGGGT GCAGAA GGT | CTAAAC CCCAAG GAAAAG TG | 443 |
| IL-8 | R | GGTGGT TTTTTC TTTTTC ATGGA | ACCAAG GCGCAG TTCAAC AG | |
| IL-10 | F | CTTGTC GGAAAT GATCCA GTTTT | ND | ND |
| IL-10 | R | TCAGGC CCGTGG TTCTCA | ND | |
| IL-12p35 | F | GCCACG GTATAG AGGATT TTCCT | CTTTCT TCAAAT GCAGCA TTGG | 271 |
| IL-12p35 | R | GGGTCT GGGTGA TACAAC GAA | GGGTCT GGGTGA TACAAC GAA | |
| IL-16 | F | GAGGGC GGTCCC AGAAGT | GGACCT CACGCG GTTTGA | 345 |
| IL-16 | R | CTCTCT AGATGC AGTCTG TCGTTT GT | CTCTCT AGATGC AGTCTG TCGTTT GT | |
| IL-18 | F | GAAAAT GATGAA GACCTG GAATCA | GAAAAT GATGAA GACCTG GAATCA | 352 |
| IL-18 | R | AACTTG GTCATT CAATT TCGTAT GA | GTCCTG GAACAC TTCTTT GA | |
| TGF-β1 | F | CTGAGC CAGAGG CGGACT AC | CTGAGC CAGAGG CGGACT AC | 262 |
| TGF-β1 | R | TGCCGT ATTCCA CCATTA GCA | TTGCTG AGGTAG CGCCAG GAATTG | |
| TNF-α | F | CGGTGG TGGGAC TCGTAT G | CAAGTA ACAAGC CGGTAG CC | 352 |
| TNF-α | R | CTGGTT GTCTTC CAGCTT CACA | TGGAAG ACTCCT CCCTGG TA |
a
F, forward; R, reverse.
b
ND, not done.