Figure 3.

Experimental setup of the intravital fluorescence videomicroscopy workstation. The animal preparation under anesthesia is placed under an epifluorescence microscope, coupled with a mercury lamp connected to a low-light-imaging silicon-intensified target (SIT) camera that includes an image processor, associated videotimer, a digital videocassette recorder (VCR) and a video monitor. For later off-line analysis, real time videos were recorded using a digital videocassette.A: Evaluation of the initial contact fraction (%) of OT-I CD8⁺ T cells with the post capillary venules (20–60 μm diameter) of the spinal cord microvasculature of mice with EAE B: Shows the evaluation of capture and rolling fractions (%) of OT-I CD8⁺ T cells with the post capillary venules (20–60 μm diameter) of the spinal cord white matter microvasculature of mice afflicted with MOG_35-55-induced EAE.