Table 1.
Parameters analyzed by intravital microscopy (modified from[22])
| Parameter | Unit | Formula |
|---|---|---|
| Total Cellular Flux (TFx) |
min-1 |
Number of T cells that pass through a vessel during a given observation period |
| Initial Contact Flux (ICFx) |
min-1 |
Number of immune cells that interact, either by rolling or getting captured to, with the vessel wall during an observation period |
| Initial Contact Fraction (ICF) |
% |
ICF = ICFx/TFx × 100 |
| Rolling Flux (RFx) |
min-1 |
Number of immune cells that roll along the vessel wall during an observation period |
| Rolling Fraction (RF) |
% |
RF = RFx/TFx × 100 |
| Capture Flux (CFx) |
min-1 |
Number of immune cells that are captured to the vessel wall during an observation period |
| Capture Fraction (CF) |
% |
CF = CFx/TFx × 100 |
| Firm Adhesion/Field Of View (FOV) | none | Number of firmly adherent T cells per one field of view (FOV) within the spinal cord window |