TABLE 1.

Sequence and expected fragment sizes of synthetic oligonucleotides used for RT-PCR

Target mRNA	Primer sequence	Size (bp)
RANKL	5′-TACTTTCGAGCGCAGATGGAT-3′	482
	5′-GTACGCTTCCCGATGTTTCAT-3′
IL-6	5′-ATGAACAACGATGATGCACTTG-3′	490
	5′-TAAGTCAGATACCTGACAACAG-3′
OPG	5′-AAACAGCACTGCACAGTGAG-3′	489
	5′-TGGTAGGAACAGCAAACCTG-3′
β-Actin	5′-TCCTGTGGCATCCATGAAACT-3′	340
	5′-AACGCAGCTCAGTAACAGTC-3′
RANKL (real-	5′-TACTTTCGAGCGCAGATGGAT-3′	89
time PCR)	5′-ACCTGCGTTTTCATGGAGTCT-3′
IL-6 (real-time	5′-ATGAACAACGATGATGCACTTG-3′	82
PCR)	5′-TATCCAGTTTGGTAGCATCCAT-3′
OPG (real-time	5′-AAACAGCACTGCACAGTGAG-3′	108
PCR)	5′-ACACTGGGCTGCAATACACA-3′
GAPDH	5′-AACTACATGGTCTACATGTTCCA-3′	63
	5′-CCATTCTCGGCCTTGACTGT-3′