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. Author manuscript; available in PMC: 2013 Jan 31.
Published in final edited form as: Chem Biol. 2012 Mar 23;19(3):353–360. doi: 10.1016/j.chembiol.2012.01.006

Figure 4. Characterization of ddRFPs.

Figure 4

(A) Fluorescence image of E. coli expressing various proteins discussed in the text. A0.4B0.5 and A1B1 are tandem constructs.

(B) Absorbance and fluorescence emission spectra for A0.4 (dashed line; 10 μM), B0.5 (gray line; 10 μM), and the equimolar mixture of A0.4 and B0.5 (black line; 10 μM each).

(C) Absorbance and fluorescence spectra of A1 (gray line; 20 μM) and tandem ddRFP-A1B1 (black line; 20 μM) before (solid line) and after (dashed line) treatment with trypsin. B1 has baseline absorbance and fluorescence. See also Figure S3.