(A) Fluorescence image of E. coli expressing various proteins discussed in the text. A0.4B0.5 and A1B1 are tandem constructs.
(B) Absorbance and fluorescence emission spectra for A0.4 (dashed line; 10 μM), B0.5 (gray line; 10 μM), and the equimolar mixture of A0.4 and B0.5 (black line; 10 μM each).
(C) Absorbance and fluorescence spectra of A1 (gray line; 20 μM) and tandem ddRFP-A1B1 (black line; 20 μM) before (solid line) and after (dashed line) treatment with trypsin. B1 has baseline absorbance and fluorescence. See also Figure S3.