A. PIKE depletion increases seizure scores. Adult (2 to 3-month-old) wild-type (□) and PIKE −/− (■) mice were injected intraperitoneally with KA (20 mg/kg). Induced seizure after KA injection was then scored (*: P<>0.01, two-way ANOVA, n=6).
B. Representative EEG recording before and after KA (15 mg/kg) injection in wild-type animals.
C. KA-provoked more spike discharge in PIKE −/− mice. Number of spike-wave discharges that were higher than the basal levels during the EEG examination in the wild-type and PIKE −/− mice after 15 mg/kg KA injection was quantified (*: P<0.05, Student's t-test, n=4).