Figure 5. NFCT of the engineered FhuA ΔC/Δ4L protein nanopore reconstituted into various lipid bilayer systems.
(A) Representative examples of single-channel electrical recordings with FhuA ΔC/Δ4L reconstituted into a bilayer formed with either DPhPS (the upper trace) or ECTPL (the lower trace); (B) The NFCTs were analyzed at an applied transmembrane potential of +180 mV. The buffer conditions are the same as in Fig. 2.
