TABLE 2.
PCR primers used in this study
| Primer | Sequence (5′-3′) | Gene | Orien- tationa | 5′ Posi- tionb |
|---|---|---|---|---|
| A0F | AACTCAGTCATCATAACTAC | sapA | F | 1903 |
| A0R | TTCCATCATCAACTACAACA | sapA | R | 2809 |
| 0F1 | AGCTTATTACAGTGAAACTA | sapA | F | 2014 |
| 0R1 | GATCTAGCGTACCTGAAA | sapA | R | 2775 |
| A1F | GGTAGTGATGATACTGTAAA | sapA1 | F | 1953 |
| A1R | AATCCAGCAAGCTTAATCAA | sapA1 | R | 2720 |
| 1F1 | TCAAACTGCAGCTAGTAAAA | sapA1 | F | 1240 |
| 1R1 | ATTGTATCATTTCCTTCACC | sapA1 | R | 1700 |
| 2F | CTAACACTATAACCGTTACT | sapA2 | F | 2741 |
| A2R | AGCATCAACAGTGTCATTAA | sapA2 | R | 3291 |
| 2F1 | GATGATGCATTAACAATAATA | sapA2 | F | 2889 |
| 2R1 | GCAGTGTCTGGAGTAACG | sapA2 | R | 3211 |
| A3F | TTTCAACGGTGCTAAGCTTA | sapA3 | F | 2496 |
| A3R | GTCTTATGGGTGGGTTAAAAG | sapA3 | R | 2973 |
| 3F1 | TAAATGCTGCGGACTAAA | sapA3 | F | 2049 |
| 3R1 | AGATAGATCTATGGCTACAC | sapA3 | R | 2426 |
| A4F | ATCTGCGTTTGATACGATAA | sapA4 | F | 1921 |
| A4R | CTCCAAGAGTTAAATTAGCTA | sapA4 | R | 2467 |
| 4F1 | GATCTGTAGATGCGCTAA | sapA4 | F | 983 |
| 4R1 | GTGAATGAATCGTTAGCCA | sapA4 | R | 1553 |
| A5F | ATGTAAGCGTAGAGAATAAG | sapA5 | F | 2618 |
| A5R | GTAAGGCTATCTAAATCAAC | sapA5 | R | 3290 |
| 5F1 | GTTACTGCTGCTGCTAAAATAG | sapA5 | F | 2971 |
| A6F | ATCTTACAGCTATCGATATCA | sapA6 | F | 2783 |
| A6R | TTGCATCTTTTGTTGTGCTA | sapA6 | R | 3562 |
| 6R1 | CCTATGGCTATCTTATCTATA | sapA6 | R | 2961 |
| 6F1 | CTAGCGCTTCACTAAAATTAG | sapA6 | F | 2183 |
| A7F | GGACTAGAAGTAGGAAATA | sapA7 | F | 2044 |
| A7R | AAGAAGCTATAGTTGAAGCA | sapA7 | R | 2243 |
| 7F1 | ATGTGAAGGTACAAAAGGAA | sapA7 | F | 3362 |
| 7R1 | CGTCTATAAGCTGTAGATTAA | sapA7 | R | 3850 |
| MF | ACCACTAGCATCAAATCTTA | mtfB | F | 37 |
| MR | CCAACTATAACTAGATCGATA | mtfB | R | 734 |
| AR | ATCAAGATCACTAGCACTA | sapA | R | 531 |
| BR | TCAACACTACTACTATTACTA | sapB | R | 525 |
| HF | AGCAGGAGGATTTGTTGAA | Cf0009 | F | 651 |
| HR | CAATAGCATTTGCATTTGTG | Cf0009 | R | 1737 |
| IF | CTTTGTTTTGTTTATTCATTGAA | sapAx | F | −261 |
| IR | GCATTAGAATAGCATTAATACT | sapAx | R | −112 |
| AbF | GATTTTATTTTATTTTATTAAGGA | sapA(B) | F | −32 |
| AbR | TATCTACTATTTATTAAGGTTTGG | sapA(B) | R | −184 |
| FF | ACTATTAGAAATTTAGAAAGAG | sapF | F | 1268 |
| DF | AGCTGAGCTAAATACACA | Cf0031 | F | 50 |
| SF | GATTTTATTTTATTTTATTAAGGA | sap | F | −32 |
| SR | TATCTACTATTTATTAAGGTTTGG | sap | R | 185 |
| DR | CAGTTCCTTCTCTTTGATAT | Cf0031 | R | 362 |
| CR | TCTGCTTGCGTATCTATACA | sapC | R | 671 |
| TR | CGTCGTTTTCATGATCGA | Cf0020 | R | 321 |
a
F, forward; R, reverse.
b
Based on the position in the specified gene (accession no. AY211269).