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. Author manuscript; available in PMC: 2014 Jan 30.
Published in final edited form as: J Am Chem Soc. 2013 Jan 16;135(4):1617–1624. doi: 10.1021/ja312353e

Figure 2. Expression, purification and activity of HSV1-TK.

Figure 2

a) Coomassie Blue staining of SDS-PAGE and western blot analysis (anti-His antibody) of purified recombinant HSV1-TK. b) Relative phosphorylation of 5 μM of the indicated agent (dT, 1–3) in the presence of pure recombinant HSV1-TK (a), as measured with the Kinase-Glo® assay. c) CEST-spectra (solid lines) and MTRasym plots (dashed lines) obtained for the kinase reactions with (black lines) and without (gray lines) purified HSV1-TK enzyme for 1. (d) MTRasym (5ppm) values of 1 before (1+ATP) and after (1+ATP+HSV1-TK) phosphorylation (n=3). Relative phosphorylation is defined in the Experimental section.