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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Nov 29;1828(3):938–943. doi: 10.1016/j.bbamem.2012.11.023

Fig 2.

Fig 2

EDTA-solubilized Hfx. volcanii S-layer glycoprotein is lipid-modified. As described in the Materials and Methods, equivalent amounts of EDTA-solubilized (top pair of panels) and Triton X-100-solubilized (bottom pair of panels) S-layer glycoprotein were treated with 1 M KOH or not (upper and lower panels in each pair, respectively). Such analysis reveals that an entity detected as a monoisotopic [M-H]− peak at m/z 731.635, corresponding in mass to archaetidic acid, was only released from the EDTA-solubilized S-layer glycoprotein following base hydrolysis (uppermost panel; the inset shows MS/MS analysis of the m/z 731.635 peak, the chemical structure of archaetidic acid and its calculated mass).