Fig. 5.

NsPEF-evoked Ca²⁺-transients in a Ca²⁺-free medium. Cells were perfused for 3 min with 0 Ca²⁺/2 mM EGTA physiological solution before the nsPEF stimulation. The right panel shows the onset of the transients on a faster time scale. Red triangles indicate the time of nsPEF application. Each trace is the average of at least 15 cells. At 30 kV/cm, [Ca²⁺]_i increase occurred in two steps, and the second step could be abolished with 2-APB. See subsection 3.2 and Fig. 6 for more detail.