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. Author manuscript; available in PMC: 2014 Jan 30.
Published in final edited form as: J Am Chem Soc. 2013 Jan 16;135(4):1317–1329. doi: 10.1021/ja3112093

Figure 2. Time dependent replacement of amide protons with deuterons upon dilution of C99 into D2O solution.

Figure 2

The intensity ratio is for the peak intensity observed after exchange with D2O for 2 (red bars) or 16 (blue bars) h relative to a non-exchanged “time = 0” reference sample. Negative bars indicate that the peaks had disappeared completely, while the absence of either a positive or negative bar indicates that data was not measured for that site because of difficulties due to peak overlap or assignment. The known topology of C99 is shown below each graph, with helical regions indicated by large, colored rectangles and dynamic loops indicated by grey, small rectangles. The numbering given in the upper line refers to full-length APP numbering while the lower line gives Aβ numbering. Data was collected for 0.2 mM U-15N-C99 in 10% LMPG micelles at pH 6.5 and 45°C.