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. 2013 Jan 31;3:1179. doi: 10.1038/srep01179

Figure 1. Conventional pluripotency markers SSEA4 and TRA-1-81 do not discriminate between partially and fully reprogrammed hiPSCs.

Figure 1

(a) Flow cytometry analysis of hiPSC lines generated by sorting human FTc1 fibroblasts directly into 96-well plate in SMC4 media 6 weeks post infection with polycistronic reprogramming lentivirus (expressing OCT4, SOX2, and KLF4). (b) SSEA4+ TRA-1-81+ cell lines were analyzed by qRT-PCR for the endogenous expression of pluripotency markers (NANOG, OCT4 and SOX2) and transgene. Error bars represent standard deviation of duplicates. Differences in gene expression of pluripotency markers in all FTc1 cell lines are significant (p-values < 0.05) relative to the H1 ESCs sample. (c) SSEA4+ TRA-1-81+ cell lines FTc1-C9 and FTc1-C12 were stained for expression of TRA-1-81 (green) and NANOG (red) and examined under fluorescence microscopy. Nuclei were stained with DAPI (blue). Scale bar is 400 μm.