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. 2013 Jan 31;3:1179. doi: 10.1038/srep01179

Figure 2. Surface marker expression in hPSCs and somatic cells.

Figure 2

(a) Expression of indicated surface markers was compared to that of NANOG and OCT4 in an array of cell types including hPSCs (hESCs; n = 2, hiPSCs; n = 16) cultured in conventional media and on MEFs, hiPSCs cultured in SMC4 media on Matrigel (n = 11), partially reprogrammed cells cultured in SMC4 media on Matrigel (n = 6), primary adult cells (fibroblasts, n = 10; adipose stem cells, n = 1; myoblasts, n = 1), and hPSC-derived differentiated cells (embryoid bodies, n = 4; definitive endoderm, n = 1; trophectoderm, n = 1; monolayer differentiation, n = 3). Expression of indicated genes was examined by qRT-PCR, and values normalized within each set to that of hESC. (b) Expression of indicated surface markers was examined by qRT-PCR in normal tissue samples (n = 48) and results are depicted as a heat map. Distances between samples and assays are calculated for hierarchical clustering based on the ΔCT values using the Pearson's Correlation. The ΔCT range values are between -3 (High) and 15 (Low).