Table 1.

PCR primers for detection of Bifidobacterium and Lactobacillus.

Gut flora	Forward primer 5′–3′	Reverse primer 5′–3′	Size (bp)	References
Lactobacillus	AGCAGTAGGGAATCTTCCA	CACCGCTACACATGGAG	341	Armougom et al., 2009
Lactobacillus acidophilus	GAAAGAGCCCAAACCAAGTGATT	CTTCCCAGATAATTCAACTATCGCTTA	145	Wellen and Hotamisligil, 2005
Lactobacillus bulgaricus	GGRTGATTTGTTGGACGCTAG	GCCGCCTTTCAAACTTGAATC	138	Wu et al., 2010a
Lactobacillus casei	GCACCGAGATTCAACATGG	GGTTCTTGGATYTATGCGGTATTAG	122	Wu et al., 2010a
Lactobacillus rhamnosus	TGCTTGCATCTTGATTTAATTTTG	GTCCATTGTGGAAGATTCCC	317	Wu et al., 2010a
Lactobacillus plantarum	TGGATCACCTCCTTTCTAAGGAAT	TGTTCTCGGTTTCATTATGAAAAAATA	145	Haarman and Knol, 2006
Bifidobacterium	GCGTGCTTAACACATGCAAGTC	CACCCGTTTCCAGGAGCTATT	126	Armougom et al., 2009
Bifidobacterium longum	GAGACAGAAACTTTCGAAGC	GAAGTCTGTGGTATCCAATCC	112	Byun et al., 2004
Bifidobacterium breve	TTCCGCATTCGTGTTATTGA	CACATCTTCGCTATCCAGCA	279	Byun et al., 2004
Bifidobacterium adolescentis	CTCCAGTTGGATGCATGTC	CGAAGGCTTGCTCCCAGT	122	Matsuki et al., 1998
Bifidobacterium infantis	CCATCTCTGGGATCGTCGG	TATCGGGGAGCAAGCGTGA	563	Matsuki et al., 1998