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Location of the ascV gene as determined by PFGE and Southern blot analysis. (A) PFGE of S1 nuclease-treated (lane 1) and intact (lane 2) genomic plugs. (B) Southern blot analysis with ascV as a probe against the gel in panel A. (C and D) Gel showing a PacI-digested genomic DNA plug from AH-1 (C) and Southern blot with ascV as a probe against the gel in panel C.
