TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype and/or relevant propertya | Sourceb or reference |
|---|---|---|
| A. hydrophila strains | ||
| Aer-19 | O:11, virulent | Human, CDHS |
| Aer-27 | Virulent | Human, CDHS |
| Aer-184 | O:34, virulent | Human, CDHS |
| Aer-186 | O:16, virulent | Human, CDHS |
| Aer-205 | O:11, virulent | Human, CDHS |
| Aer-211 | O:18, virulent | Human, CDHS |
| ATCC 7966 | O:1, type strain | Milk, ATCC |
| AH-1 | O:11, virulent, Colr Cms | Fish, UM |
| aopB mutant | aopB insertion mutant from AH-1 | This study |
| aopD mutant | aopD insertion mutant from AH-1 | This study |
| AH-3 | O:34, virulent | Fish, UB |
| Ba5 | O:34, virulent | Fish, UM |
| JCM3968 | O:6 | Snake, JCM |
| JCM3973 | O:11 | Unknown, JCM |
| JCM3976 | O:14 | Frog, JCM |
| JCM3978 | O:16 | Unknown, JCM |
| JCM3980 | O:18 | Unknown, JCM |
| JCM3981 | O:19 | Unknown, JCM |
| JCM3983 | O:21 | Unknown, JCM |
| JCM3984 | O:22 | Unknown, JCM |
| JCM3985 | O:23 | Unknown, JCM |
| JCM3996 | O:34 | Unknown, JCM |
| L15 | O:51, avirulent | Fish, BAU |
| L31 | O:91, virulent | Fish, BAU |
| L36 | O:36, avirulent | Fish, BAU |
| LL1 | O:11, virulent | Fish, UM |
| PPD35/85 | O:7, avirulent | Fish, AVA |
| PPD11/90 | O:21, virulent | Fish, AVA |
| PPD64/90 | O:34, avirulent | Fish, AVA |
| PPD88/90 | O:16, avirulent | Fish, AVA |
| PPD45/91 | Avirulent | Fish, AVA |
| PPD70/91 | O:5, virulent | Fish, AVA |
| PPD122/91 | O:11, virulent | Fish, AVA |
| PPD134/91 | O:18, virulent | Fish, AVA |
| Xs91/4/1 | Virulent | Fish, China |
| E. coli strains | ||
| JM109 | Cols Amps | Promega |
| MC1061(λpir) | (λpir) thi thr-1 leu6 proA2 his-4 argE2 lacY1 galK2 ara14 xyl5 supE44, λpir | 34 |
| S17-1(λpir) | thi pro hsdR hsdM+recA[RP42-Tc::Mu-Km::Tn7 (Tpr Smr)Tra+] | 37 |
| Plasmids | ||
| pGEM-T Easy vector | Cloning vector; Apr | Promega |
| pFS100 | pGP704 suicide plasmid, λpir dependent; Kmr | 34 |
| pCM100 | pGP704 suicide plasmid, λpir dependent; Cmr | This study |
| pCM-AOPB | pCM with an internal fragment of aopB (605 bp) | This study |
| pCM-AOPD | pCM with an internal fragment of aopD (565 bp) | This study |
a
Virulent strains were defined as having a lower LD50 in blue gourami or rainbow trout (<106.5) than the avirulent strains (>107.5).
b
ATCC, American Type Culture Collection; AVA, Agri-Food and Veterinary Authority, Singapore; BAU, Bogor Agricultural University of Indonesia; CDHS, California Department of Health Services; JCM, Japan Collection of Microorganisms; UB, University of Barcelona; UM, University of Montreal.