Figure 3.

AMD3100 increases capillary density and the number of BM-derived endothelial cells in the myocardium after IR injury. AMD3100 or saline was subcutaneously injected after IR injury in WT mice that had been transplanted with BM from GFP-expressing mice. (A, B) BM-derived (i.e., GFP-expressing) cells (green) were identified in the AAR and quantified on day 1, day 3, and day 28 after injury; scale bar=100 um. (C–E) On day 28 after IR injury, mice were perfused with BS1-lectin before sacrifice, and sections from the AAR were stained with fluorescent anti-lectin antibodies. (C) BM-derived endothelial cells were quantified as the number of cells positive for both GFP expression and lectin staining; scale bar=100 um. (D) BM-derived cells (green, GFP fluorescence) and functional vascular structures (red, lectin fluorescence) were identified in the AAR, and (E) capillary density was quantified as the number of lectin+ vascular structures. *P<0.05 and **P<0.01 versus saline. Panel B: n=3–6 per treatment group at each time point; Panels D, E: n=3–4 per treatment group.