FIG. 1.

Competition for binding to rMSP-1₁₉ between mouse MAbs and IgG antibodies in human sera from The Gambia. Competition assays were performed at a serum dilution of 1:50. Anti-MSP-1₁₉ ODs were determined at a serum dilution of 1:1,000. A total of 236 Gambian sera were tested. (a and b) Correlation between anti-MSP-1₁₉ OD and percent competition with MAb 12.10 (a) and MAb 8A12 (b). (c) Five sera which gave a low anti-MSP-1₁₉ OD at 1:1,000 but showed >50% competition with MAb 12.10 at 1:50 were serially titrated. The titrated sera are indicated in the inset (which is a detail from panel a). Serum 1220, which showed <50% competition with MAb 12.10 (and thus is not shown in the inset), was titrated as a negative control. O, serum 1254; ▪, serum 1073; •, serum 1052; ▴, serum 1176; x, serum 1252; □, 1220; ◊, positive control serum pool.