FIG. 4.

Correlation of binding of human serum IgG to wild-type rMSP-1₁₉ and rMSP-1₁₉ mutants. IgG ODs for sera at a 1:1,000 dilution were determined with an ELISA. (a) Wild-type rMSP-1₁₉ versus M4X34 for 55 Gambian sera. (b) M4X15 versus M4X34 for 55 Gambian sera. (c) Wild-type rMSP-1₁₉ versus M4X34 for 56 Ugandan sera. (d) M4X15 versus M4X34 for 56 Ugandan sera. The diagonal lines indicate the distributions of values expected if antibodies bind equally to the proteins. Binding to different mutant proteins (b and d) was highly correlated, but a poor correlation was observed for binding to wild-type and mutant proteins (a and c) for some sera.