Table 1. Yersinia pestis antigen PCR primer sequences.
| Primer | PCR Primer Sequencea | RE Site |
|---|---|---|
| 5′ F1/IRES/tPA | 5′-cgcgcGTCGACgaggtaatatatgaaaaaaatcag-3′ | Sal I |
| 5′ F1 | 5′-gtgaGTCGACatgaaaaaaatcagttccgttatc-3′ | Sal I |
| 3′ F1 | 5′-gcGAATTCttattggttagatacggttacggt-3′ | EcoR I |
| 5′ V | 5′-gtgaGTCGACatgattagagcctacgaacaaaacc-3′ | Sal I |
| 5′ V/IRES/tPA | 5′-tgacGCCGGCattagagcctac-3′ | NgoM IV |
| 3′ V | 5′-cgcGAATTCtcatttaccagacgtgtcatc-3′ | EcoR I |
| 3′ V307 | 5′-gcGAATTCtcaacggttcagtgcttcaatag-3′ | EcoR I |
a
Restriction enzyme sites are capitalized.