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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Arch Virol. 2012 Jun 8;157(9):1803–1806. doi: 10.1007/s00705-012-1367-y

Figure 1.

Figure 1

ORF A gene expression. (A) Detection of ORF A and ORF 4 transcripts in SVV-infected Vero cells by Northern blot hybridization using DIG-labeled ORF A and ORF 4 DNA probes, respectively. (B) Activation of the ORF A promoter in SVV-infected Vero cells as indicated by transfection of a ORF A promoter-pGL3 construct into SVV-infected or uninfected Vero cells. (C) SVV IE62-mediated transactivation of the ORF A promoter. Vero cells were transfected with the pCI vector, the ORF A pr-GL3 plus the pCI vector, or with ORF A pr-pGL3 plus pCI-IE62. (D) Deletion analysis of the ORF A promoter. Vero cells were transfected with the ORF A pr-pGL3 or ORF A deletion mutants plus pCI-IE62. In all cases luciferase activity was detected in cell lysates at 48 hrs post-transfection. Experiments were conducted in triplicate. Fold activation is relative to the Vero (B) or pCI (C and D) transfected controls. Statistical P values are indicated. NS- statistically not significant.