Transactivation assays. (A) Transactivation of the SVV ORF 29 promoter by SVV IE-62, but not ORF A. Vero cells were co-transfected with the ORF29pr-pGL3 and also with pCI, pCI-ORFA, pCI-ORF4, or pCI-62. (B) Stimulation of SVV IE62 mediated transactivation of the ORF 29 promoter. Vero cells were co-transfected with the ORF29pr-pGL3 and pCI-IE62 and also with pCI-ORFA, or pCI-ORF4. In all cases luciferase activity was detected in cell lysates at 48 hrs post-transfection. Experiments were conducted in triplicate. Fold activation is relative to the pCI transfected controls. Statistical P values are indicated. NS- statistically not significant.