Figure 5.

Cell surface TCR-α/-β expression in CD4⁺ T cells is not affected by age. Confocal microscopy of TCR-α/-β expression in CD4⁺ T cells from young and elderly donors that were either left untreated (vehicle control) or pre-treated with blebbistatin. Representative data are from one donor pair out of 10 pairs tested. The fluorescence intensity of TCR-α/-β in the plasma membrane was performed on the complete three-dimensional confocal stack for 30 cells obtained from a minimum of five different fields each, from a minimum of 10 independent experiments. TCR, T-cell receptor.