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. Author manuscript; available in PMC: 2013 Jan 31.

Published in final edited form as: Mol Ther. 2008 Jun 17;16(8):1409–1416. doi: 10.1038/mt.2008.116

A group of 100 isolated islets were transduced with dsAAV8 expressing either GFP, or mIL-4 under mIP and subsequently cultured in RPMI for 6 days and the mIL-4 secreted into media was assayed by ELISA. The mIL-4 release in eGFP and AAV8-mIL-4 transduced islets on day 4 and 6 of cultured media (a) and in the islet lysate on day 6 (b) respectively post-transduction and culture. Secretion of mIL-4 from dsAAV8mIP-eGFP and mIL-4 in vivo transduced NOD islets. 4X10¹¹ v.g. of dsAAV8-mIP expressing either eGFP or mIL-4 was injected i.p. and the islets subsequently isolated as described in methods. A group of 100 isolated islets were cultured in RPMI for 48 hr and the levels of mIL-4 in the media were determined as above. The mIL-4 release in eGFP (control) and mIL-4 (2c) transduced islets in 48 h culture media. The data represent the mean ± SEM of two independent experiments in triplicate (* p<0.001).

A group of 100 isolated islets were transduced with dsAAV8 expressing either GFP, or mIL-4 under mIP and subsequently cultured in RPMI for 6 days and the mIL-4 secreted into media was assayed by ELISA. The mIL-4 release in eGFP and AAV8-mIL-4 transduced islets on day 4 and 6 of cultured media (a) and in the islet lysate on day 6 (b) respectively post-transduction and culture. Secretion of mIL-4 from dsAAV8mIP-eGFP and mIL-4 in vivo transduced NOD islets. 4X10¹¹ v.g. of dsAAV8-mIP expressing either eGFP or mIL-4 was injected i.p. and the islets subsequently isolated as described in methods. A group of 100 isolated islets were cultured in RPMI for 48 hr and the levels of mIL-4 in the media were determined as above. The mIL-4 release in eGFP (control) and mIL-4 (2c) transduced islets in 48 h culture media. The data represent the mean ± SEM of two independent experiments in triplicate (* p<0.001).