Figure 2. DNA binding is not significantly affected by mutations.

(A) Amalgamated gel from representative experiments showing DNA binding of WT and mutants at 0, 12.5, 25, 50 and 100 nM protein. (B) Binding data compiled from triplicate experiments. Mutants universally appear to have slightly reduced binding compared to wild-type, but the difference is not significant. Error bars represent standard error of mean from three experiments.