TABLE 2.

Tissue-specific constitutive expression of MsNramp

Tissue	Cp for controls (avg ± SEM)a	PCREb	Fold difference compared with white musclec
Anterior kidney	21.01 ± 0.29	1.72	85
PE cells	23.54 ± 0.18	1.80	28
Spleen	18.90 ± 0.32	1.70	240
White muscle	29.20 ± 0.32	1.72	NA

^aThe average crossing point (Cp) was calculated from nine control fish (three fish per time point); the crossing point is the cycle during amplification (45 total cycles) at which fluorescence rises above the background level. The values for all tissues types are significantly different (P < 0.01).

^bPCR_E is the efficiency of the PCR for each tissue type.

^cThe fold difference was calculated by using PCR_E^ΔCp. NA, not applicable.