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(A) LCs were isolated using AutoMACS with anti-MHCII-PE and anti-PE microbeadsfollowed by a cell sorter. The purity was confirmed to be ~98%.(B) Expression profiles of miRNAs in young and aged LCs from two individual experiments. (C) miRNA expression in LCs was significantly up- and downregulated in aged mice compared to that in young mice. (D) Changed miRNAs in aging were confirmed by single TaqMan RT-PCR.
