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. 2004 Mar;72(3):1725–1732. doi: 10.1128/IAI.72.3.1725-1732.2004

FIG. 4.

FIG. 4.

r-Fim of P. gingivalis also induces MDDCs to become immunostimulatory. (A) FACS analysis demonstrating upregulation in MFI of, from left to right, HLA-DR, CD80, CD83, and CD86 on MDDCs pulsed with P. gingivalis r-Fim at 10 μg/ml for 18 h or not pulsed with r-Fim. ctl, isotype controls. (B) FACS analysis of IFN-γ, TNF-α, and IL-10 released from MDDCs pulsed with r-Fim (RFIM10) or not pulsed (DC CONT) for 18 h. DC supernatants were analyzed by flow cytometry using the cytometric bead assay (CBA kit; BD Biosciences) as for Fig. 3. (C) MDDCs pulsed with 10 μg of r-Fim/ml for 18 h (RFM10Ugm) or not pulsed with r-Fim (DC CONT) were cocultured in graded doses (5,000, 1,000, and 300 DCs) with 50,000 autologous lymphocytes in AB serum and RPMI for 5 days, and uptake of tritiated thymidine was analyzed. (D) T-cell cytokines. Fifty thousand autologous CD4+ T cells were cocultured with 5,000 MDDCs in AB serum and RPMI, and T-cell supernatants were analyzed for IFN-γ, TNF-α, IL-10, and IL-2 by FACS analysis (CBA kit; BD Biosciences).