Figure 1.

Western blot of standards and plasma samples immunostained with the total-tau antibody employed in the total-tau quantification ELISA kits produced by Invitrogen. Molecular weight standards are in lane 1, tau isoform standards are in lane 2, and authentic human 441 tau is seen in lane 4. No sample (blanks) was added to lanes 3, 5, 6, 8, 10, 12 and 14. A plasma sample - after stripping IgG - from an Alzheimer’s patient was applied to lane 7, from an individual with MCI to lane 9, from a control subject to lane 11, from a rabbit fed control diet to lane 13 and from a rabbit fed a 1% cholesterol diet to lane 15.