Table 1.
TaqMan genotyping assays used to genotype APOE, MAPT, and PSEN1
| Gene (position) | dbSNP ID | ABI TaqMan assay | Direction | PCR primers | Reporter primers (dye) |
|---|---|---|---|---|---|
| APOE 526T>C (p.Cys176Arg) | rs7412 | C_904973_10 | N/A | N/A | N/A |
| APOE 388C>T (p.Arg130Cys) | rs429358 | C_3084793_20 | N/A | N/A | N/A |
| MAPT 1216C>T (p.Arg406Trp) | rs63750424 | C_27537056_1 0 | N/A | N/A | N/A |
| PSEN1 653C>T (p.Pro218Leu) | NA | Custom assay | Forward Reverse | GAATTTTGGTGTGGTGGGAATGATT GCCATGAGGGCACTAATCATAATGA | AGTCGAAGTGGACCTTT (VIC) AGTCGAAGTAGACCTTT (FAM) |
APOE, apolipoprotein E, PSEN1, presenilin 1, PSEN2, presenilin 2, N/A, not applicable.