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. 2012 Jul 23;1(2):130–145.

Figure 4.

Figure 4

Protein-protein interactions in nuclear-enriched fractions from human lumbar spinal cord. Protein extracts from ALS and controls were prepared as described in the Materials and Methods. Protein (500 μg) from each extract was immunoprecipitated with CRABP-II and resulting blots were probed with antibodies specific to RARα (A) or RARβ (B). A: Immunoblots of nuclear-enriched fractions from lumbar spinal cord co-immunoprecipitated with CRABP-II and probed for RARα. B: Immunoblots of nuclear-enriched fractions from lumbar spinal cord coimmunoprecipitated with CRABP-II and probed for RARβ. C: Graph representing the results from densitometry quantification. RAR Receptor levels were normalized to that of CRABP-II for each sample. Black bars represent control cases (n = 2) and checkered bars are ALS cases (n = 6). Statistical analyses were performed using an unpaired t-test with a 95% confidence interval. The p values for RARα and RARβ were 0.9042 and 0.8881, respectively. Letters and numbers correspond to patients in Table 1.